HSP70 Antibody: FITC

• Catalog Number: orb376901
• Category: Antibodies
• Description: Mouse monoclonal antibody against HSP70 conjugated to FITC.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C92F3A-5
• Tested applications: ICC, IF, IHC
• Reactivity: Bovine, Canine, Drosophila, Fish, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Other, Porcine, Rabbit, Rat, Sheep
• Isotype: IgG1
• Immunogen: Human HSP70
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), IHC (1:10000), ICC/IF (1:1000), FACS (1:1000)
• Conjugation: FITC
• MW: 70kDa
• Target: HSP70
• Entrez: 3303
• UniProt ID: P0DMV9, P0DMV8
• NCBI: NP_005336.3
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36mM Ethanolamine, and 9.09mM Sodium Bicarbonate in 90.9% PBS
• Alternative names: HSP70 1 antibody, HSP70 2 antibody, HSP70.1 antibo
• Note: For research use only
• Application notes: 1 µg/ml of SMC-100 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5. Tissue: colon carcinoma. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5. Tissue: Heat Shocked Melanoma cells. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 16 hours at RT. Secondary Antibody: Biotin Goat Anti-Mouse.

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92F3A-5. Tissue: Heat Shocked CD3+ CD8+ T cells. Species: Mouse. Primary Antibody: Mouse Anti-Hsp70: FITC Monoclonal Antibody at 1:1000.

Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.