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HSP70 Antibody (Acetyl Lys77): Biotin
Catalog Number: orb414154
| Catalog Number | orb414154 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody against HSP70 Acetyl Lys77 conjugated to Biotin |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, ICC, IF, IHC, WB |
| Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide from the N-terminal of Human HSP70 Acetyl Lys77 (1-100 aa), conjugated to Keyhole Limpet Haemocyanin (KLH). |
| Concentration | 0.5mg/mL |
| Dilution range | WB (1:1000); ELISA (1:1000); ICC/IF (1:60); IHC (1:50) |
| Conjugation | Biotin |
| MW | 70 kDa |
| Target | HSP70 Acetyl Lys77 |
| Entrez | 3303 |
| UniProt ID | P0DMV9, P0DMV8 |
| NCBI | NP_005336.3 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS |
| Alternative names | HSP70 Acetylated lysine 77 antibody, HSP70 acLys77 Read more... |
| Note | For research use only |
| Application notes | A 1:1000 dilution of SPC-743 was sufficient for detection of HSP70 Acetyl Lys77 in 10 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody. Tissue: Embryonic kidney cells (HEK293) cultured overnight with 50 μM H2O2. Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody at 1:60 for 30-60 min at RT. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) HSP70 Antibody (Acetyl Lys77). (D) Composite.
Immunohistochemistry analysis using Rabbit Anti-HSP70 (Acetyl Lys77) Polyclonal Antibody. Tissue: Colon Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-HSP70 (Acetyl Lys77) Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~70 kDa HSP70 Acetyl Lys77 protein using Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Lane 3: H2O2 Cervical Cancer cell line (HeLa) lysate. Load: 10 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-HSP70 Acetyl Lys77 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~70 kDa.
