HSP60 Rabbit Polyclonal Antibody

• Catalog Number: orb10846
• Category: Antibodies
• Description: HSP60 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HSP60 (501-573/573aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 58 kDa
• Target: HSPD1
• UniProt ID: P10809
• RRID: AB_10747037
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: HSP 60; HSP-60; 60 kDa chaperonin;60 kDa heat shoc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (HSP60) polyclonal Antibody, Unconjugated (orb10846) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HSP60) Polyclonal Antibody, Unconjugated (orb10846) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Liver (Mouse) Lysate at 40 ug, Lane 2: Adrenal gland (Mouse) Lysate at 40 ug, Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 4: Adrenal gland (Rat) Lysate at 40 ug, Lane 5: Jurkat (Human) Cell Lysate at 30 ug, Lane 6: MDA-MB-231 (Human) Cell Lysate at 30 ug, Lane 7: HepG2 (Human) Cell Lysate at 30 ug, Lane 8: Hela (Human) Cell Lysate at 30 ug, Lane 9: K562 (Human) Cell Lysate at 30 ug, Primary: Anti-HSP60 (orb10846) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62 kD, Observed band size: 62 kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-HSP60 Polyclonal Antibody, Unconjugated (orb10846) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-HSP60 Polyclonal Antibody, Unconjugated (orb10846) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.