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Catalog Number | orb606403 |
---|---|
Category | Antibodies |
Description | HSP60 has been implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt] |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | HSPD1/2206R |
Tested applications | IHC-P, WB |
Reactivity | Canine, Human |
Isotype | Rabbit IgG, kappa |
Immunogen | Recombinant human protein was used as the immunogen for the recombinant HSP60 antibody. |
Dilution range | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Purity | Protein A affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This recombinant HSP60 antibody is available for research use only. |
UniProt ID | P10809 |
Storage | Store the recombinant HSP60 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the recombinant HSP60 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human liver tissue with recombinant HSP60 antibody (clone HSPD1/2206R). HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human HeLa lysate with recombinant HSP60 antibody (clone HSPD1/2206R). Predicted molecular weight: ~60 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant HSP60 antibody (clone HSPD1/2206R) as confirmation of integrity and purity.
IHC testing of FFPE human liver tissue with recombinant HSP60 antibody (clone HSPD1/2206R). HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE dog pancreas tissue with recombinant HSP60 antibody (clone HSPD1/2206R). HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF, IHC, WB | |
Bovine, Canine, Drosophila, Frog, Gallus, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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