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    HSP60 Antibody

    Catalog Number: orb606403

    DispatchUsually dispatched within 5-10 working days
    $ 764.00
    Catalog Numberorb606403
    CategoryAntibodies
    DescriptionHSP60 has been implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt]
    Species/HostRabbit
    ClonalityRecombinant
    Clone NumberHSPD1/2206R
    Tested applicationsIHC-P, WB
    ReactivityCanine, Human
    IsotypeRabbit IgG, kappa
    ImmunogenRecombinant human protein was used as the immunogen for the recombinant HSP60 antibody.
    Dilution rangeWestern blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
    PurityProtein A affinity chromatography
    ConjugationUnconjugated
    Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
    Hazard InformationThis recombinant HSP60 antibody is available for research use only.
    UniProt IDP10809
    StorageStore the recombinant HSP60 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
    Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
    NoteFor research use only
    Application notesOptimal dilution of the recombinant HSP60 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
    Expiration Date12 months from date of receipt.
    HSP60 Antibody

    IHC testing of FFPE human liver tissue with recombinant HSP60 antibody (clone HSPD1/2206R). HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

    HSP60 Antibody

    Western blot testing of human HeLa lysate with recombinant HSP60 antibody (clone HSPD1/2206R). Predicted molecular weight: ~60 kDa.

    HSP60 Antibody

    SDS-PAGE analysis of purified, BSA-free recombinant HSP60 antibody (clone HSPD1/2206R) as confirmation of integrity and purity.

    HSP60 Antibody

    IHC testing of FFPE human liver tissue with recombinant HSP60 antibody (clone HSPD1/2206R). HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

    HSP60 Antibody

    IHC testing of FFPE dog pancreas tissue with recombinant HSP60 antibody (clone HSPD1/2206R). HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

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