HSP60 Antibody

• Catalog Number: orb1822503
• Category: Antibodies
• Description: Mouse Anti-Human HSP60 Monoclonal IgG1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LK2
• Tested applications: FC, IHC, WB
• Reactivity: Bacteria, Bovine, Canine, E. coli, Fish, Gallus, Guinea pig, H. pylori, Hamster, Human, Insect, Monkey, Mouse, Other, Plant, Porcine, Rabbit, Rat, Yeast
• Isotype: IgG1
• Immunogen: Recombinant human HSP60
• Concentration: 1 mg/ml
• Dilution range: WB (1:4000), IHC (1:100); optimal dilutions for assays should be determined by the user.
• Conjugation: Unconjugated
• MW: 60kDa
• Target: HSP60
• Entrez: 3329
• UniProt ID: P10809
• NCBI: NP_002147.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Alternative names: CPN60 Antibody, GROEL Antibody, HLD4 Antibody, HSP
• Note: For research use only
• Application notes: 0.25 µg/ml of SMC-111 was sufficient for detection of HSP60 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-2. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-2. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining.

Immunohistochemistry analysis using Mouse Anti-HSP60 Monoclonal Antibody, Clone LK2. Tissue: Kidney. Species: Human. Fixation: Formalin Fixed, Paraffin Embedded. Primary Antibody: Mouse Anti-HSP60 Monoclonal Antibody.

Western Blot analysis of Human Heat Shocked cervical cancer cell line (HeLa) lysate showing detection of Hsp60 protein using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-2. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.