HSP60 antibody

• Catalog Number: orb1151535
• Category: Antibodies
• Description: Mouse monoclonal antibody to HSP60
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rHSPD1/6497
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human full-length HSP60 protein was used as the immunogen for the recombinant HSP60 antibody. Its epitope is localized between amino acids 383-447 of human hsp60.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant HSP60 antibody is available for research use only.
• UniProt ID: P10809
• Storage: Aliquot the recombinant HSP60 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant HSP60 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human 1) Hep-2 and 2) HeLa cell lysate using recombinant HSP60 antibody (clone rHSPD1/6497). Predicted molecular weight: ~60 kDa.

Western blot testing of human Hep-2 cell lysate using recombinant HSP60 antibody (clone rHSPD1/6497). Predicted molecular weight: ~60 kDa.

IHC staining of FFPE human kidney tissue with recombinant HSP60 antibody (clone rHSPD1/6497). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant HSP60 antibody (clone rHSPD1/6497) as confirmation of integrity and purity.