HSP60 antibody

• Catalog Number: orb1151529
• Category: Antibodies
• Description: Rabbit monoclonal antibody to HSP60
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: HSPD1/6498R
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG, kappa
• Immunogen: Recombinant human full-length HSP60 protein was used as the immunogen for the HSP60/GROEL antibody. Its epitope is localized between amino acids 383-447 of human hsp60.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This HSP60/GROEL antibody is available for research use only.
• UniProt ID: P10809
• Storage: Aliquot the HSP60/GROEL antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the HSP60/GROEL antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human 1) Hep2 and 2) HeLa cell lysate using HSP60/GROEL antibody (clone HSPD1/6498R). Predicted molecular weight: ~60 kDa.

Western blot testing of human Hep2 cell lysate using HSP60/GROEL antibody (clone HSPD1/6498R). Predicted molecular weight: ~60 kDa.

IHC staining of FFPE human liver tissue with HSP60/GROEL antibody (clone HSPD1/6498R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.