HSP60 Antibody: Biotin

• Catalog Number: orb151275
• Category: Antibodies
• Description: Rabbit polyclonal to Hsp60 (Biotin). In both prokaryotic and eukaryotic cells, the misfolding In both prokaryotic and eukaryotic cells, the misfolding...
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Bovine, Canine, Gallus, Hamster, Human, Mouse, Rabbit, Rat
• Immunogen: Human HSP60 produced through recombinant DNA methods in E.coli
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Conjugation: Biotin
• MW: 60kDa
• Target: HSP60
• Entrez: 3329
• UniProt ID: P10809
• NCBI: NP_002147.2
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: CPN60 antibody, GROEL antibody, HLD4 antibody, HSP
• Note: For research use only
• Application notes: 1 µg/ml of SPC-105 was sufficient for detection of HSP60 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp60 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp60 Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion matrix. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp60 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Immunohistochemistry analysis using Rabbit Anti-HSP60 Polyclonal Antibody. Tissue: Adrenal. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody.

Western blot analysis of Human, Dog, Mouse SKBR3, MDCK, and MEF cell line lysates showing detection of HSP60 protein using Rabbit Anti-HSP60 Polyclonal Antibody. Lane 1: Recom. Human Hsp60 (100ng), Lane2, 3 and 4: SKBR3 lysate (human), MDCK lysate (dog) and MEF lysate (mouse) (al at 7.5ug). Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody at 1:1000.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp60 Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp60 Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion matrix. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp60 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.