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Catalog Number | orb234371 |
---|---|
Category | Antibodies |
Description | Hsp47/SERPINH1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human Hsp47 recombinant protein (Position: D247-L418). Human Hsp47 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp47. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 46441 MW |
UniProt ID | P50454 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Serpin H1;47 kDa heat shock protein;Arsenic-transa Read more... |
Note | For research use only |
Application notes | WB: The detection limit for Hsp47 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody.Lane 1:human A431 cell; 2:human SK-N-SH cell; 3:human HeLa cell; 4:rat lung tissue; 5:rat kidney tissue; 6:rat L6 cell; 7:mouse lung tissue; 8:mouse NIH/3T3 cell.
IHC analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody. Hsp47/SERPINH1 was detected in a paraffin-embedded section of human breast cancer tissue.
IHC analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody. Hsp47/SERPINH1 was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue.
IHC analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody. Hsp47/SERPINH1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue.
IHC analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody. Hsp47/SERPINH1 was detected in a paraffin-embedded section of human liver cancer tissue.
IHC analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody. Hsp47/SERPINH1 was detected in a paraffin-embedded section of human spleen tissue.
IHC analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody. Hsp47/SERPINH1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue.
IF, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, WB | |
Human, Mouse | |
Polyclonal | |
Unconjugated |
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