HSP40 Antibody (FITC)

SKU: orb147603

Description

Mouse monoclonal to Hsp40, YDJ1 (FITC). Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of Hsp70 function. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins. The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1. Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. ..

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution range: WB (1:2000)
• Reactivity: Yeast
• Application Notes:
  0.5 µg/ml of SMC-166 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone No.: 2A7.H6
• Immunogen: Full length protein HSP40 (YDJ1)
• Target: HSP40
• Molecular Weight: 40kDa
• Purification: Protein G Purified

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

DNAJB1, HSP40, HSPF1, HDJ1, DNAJ1, DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, Heat shock 40 kDa protein 1, Human DnaJ protein 1, hDj-1, heat shock protein 40

Western Blot analysis of Yeast recombinant cell lysate showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 2A7.H6. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Quick Database Links

Gene Symbol

HSP40

UniProt

P25491

NCBI Gene ID

855661

RefSeq (Protein):NP_014335.1