HSP40, YDJ1 Antibody: Biotin

• Catalog Number: orb147330
• Category: Antibodies
• Description: Mouse monoclonal to Hsp40, YDJ1 (Biotin). Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain. There are two main types of Hsp40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. , type II does not. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. Hsp40 stimulates the ATPase activity of Hsp70 which in turn causes conformational changes of the unfolded proteins . The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1G10.H8
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Yeast
• Isotype: IgG1 Kappa
• Immunogen: Full length protein yeast HSP40 (YDJ1)
• Concentration: 1 mg/ml
• Dilution range: WB (1:2000)
• Conjugation: Biotin
• MW: 40kDa
• Target: HSP40
• Entrez: 855661
• UniProt ID: P25491
• NCBI: NP_014335.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: DNAJA2 antibody, CPR3 antibody, HIRIP4 antibody, D
• Note: For research use only
• Application notes: 0.5 µg/ml of SMC-150 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of Yeast Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 1G10.H8. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.