HSP27 (phospho-S82) antibody

• Catalog Number: orb338948
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HSPB1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S82 of human HSP27 protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HSPB1
• Entrez: 15507, 3315
• UniProt ID: P14602, P04792
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti HSP27 antibody, anti HSP28 antibody, anti Hea
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HSP27 (Phospho-S82) expression in A549 (A), HepG2 (B), K562 (C) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 27 kD)

Immunohistochemical analysis of HSP27 (Phospho-S82) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.