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Catalog Number | orb1151565 |
---|---|
Category | Antibodies |
Description | Rabbit monoclonal antibody to HSP27 |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | HSPB1/6490R |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | Recombinant human full-length HSP27 protein was used as the immunogen for the recombinant HSP27 antibody. |
Dilution range | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein A/G affinity |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This recombinant HSP27 antibody is available for research use only. |
UniProt ID | P04792 |
Storage | Aliquot the recombinant HSP27 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the recombinant HSP27 antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
Western blot testing of human liver tissue lysate using recombinant HSP27 antibody (clone HSPB1/6490R). Predicted molecular weight: 23-27 kDa.
IHC staining of FFPE human thyroid with recombinant HSP27 antibody (clone HSPB1/6490R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant HSP27 antibody (clone HSPB1/6490R) as confirmation of integrity and purity.
FACS, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IHC-P, WB | |
Bovine, Canine, Porcine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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