HSP27 Antibody / HSPB1

• Catalog Number: orb749572
• Category: Antibodies
• Description: It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HSPB1/774
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human HSPB1 protein was used as the immunogen for the HSP27 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This HSP27 antibody is available for research use only.
• UniProt ID: P04792
• Storage: Store the HSP27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the HSP27 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human MCF7 cells with HSP27 antibody (clone HSPB1/774, green) and Reddot nuclear stain (red).

IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP27 antibody (clone HSPB1/774).

IHC: Formalin-fixed, paraffin-embedded human prostate carcinoma stained with HSP27 antibody (clone HSPB1/774).

Flow cytometry testing of PFA-fixed human MCF7 cells with HSP27 antibody (clone HSPB1/774); Red=isotype control, Blue=HSP27 antibody.

SDS-PAGE analysis of purified, BSA-free HSP27 antibody (clone HSPB1/774) as confirmation of integrity and purity.

Western blot testing of human HeLa cell lysate with HSP27 antibody. Expected molecular weight: 23~27 kDa.