HSP27 Antibody / HSPB1

• Catalog Number: orb606902
• Category: Antibodies
• Description: HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian -crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore, HSP27 may be useful in classifying various hormone sensitive tumors.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CPTC-HSPB1-2
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Full length recombinant human protein was used as the immunogen for the HSP27 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This HSP27 antibody is available for research use only.
• UniProt ID: P04792
• Storage: Store the HSP27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the HSP27 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human prostate tissue with HSP27 antibody (clone CPTC-HSPB1-2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Immunofluorescent staining of PFA-fixed human MCF7 cells with HSP27 antibody (clone CPTC-HSPB1-2, green) and Reddot nuclear stain (red).

Flow cytometry testing of PFA-fixed human MCF7 cells with HSP27 antibody (clone CPTC-HSPB1-2); Red=isotype control, Blue= HSP27 antibody.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using HSP27 antibody (clone CPTC-HSPB1-2). These results demonstrate the foremost specificity of the CPTC-HSPB1-2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free HSP27 antibody (clone CPTC-HSPB1-2) as confirmation of integrity and purity.

Western blot testing of human liver tissue lysate with HSP27 antibody (clone CPTC-HSPB1-2). Predicted molecular weight: 23-27 kDa.