Hsp 60 Antibody (N-term)

• Catalog Number: orb1929796
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB19532
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Dilution range: WB: 1:2000, WB: 1:2000, WB: 1:1000, IHC-P: 1:50~100, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 54794 Da
• Target: This Hsp 60 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-109 amino acids from the N-terminal region of human Hsp 60.
• UniProt ID: Q0VDF9
• NCBI: NP_057383.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Heat shock 70 kDa protein 14, HSP70-like protein 1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Staining Hsp 60 in human pancreas sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Formalin-fixed and paraffin-embedded human lung carcinoma with Hsp 60 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of Hsp 60 Antibody (N-term) in mouse lung tissue lysates (35 ug/lane). Hsp (arrow) was detected using the purified Pab.

All lanes: Anti-Hsp 60 Antibody (N-term) at 1:2000 dilution. Lane 1: K562 whole cell lysate. Lane 2: 293T/17 whole cell lysate. Lane 3: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-Hsp 60 Antibody (N-term) at 1:2000 dilution. Lane 1: Raji whole cell lysates. Lane 2: K562 whole cell lysates. Lane 3: Jurkat whole cell lysates. Lane 4: Hela whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.