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Catalog Number | orb32768 |
---|---|
Category | Antibodies |
Description | Peptide affinity purified rabbit polyclonal antibody. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB34568 |
Tested applications | WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | Synthetic Peptide |
Dilution range | WB: 1:1000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 42252 |
Target | HSD3B1 |
UniProt ID | P14060 |
NCBI | NP_000853.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Anti-3 beta-hydroxysteroid dehydrogenase/Delta 5-- Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
HSD3B1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35 ug/lane). This demonstrates the HSD3B1 antibody detected the HSD3B1 protein (arrow).
Western blot analysis of HSD3B1 (arrow) using rabbit polyclonal HSD3B1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSD3B1 gene.
Anti-HSD3B1 Antibody (N-term) at 1:1000 dilution + human placenta lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.