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HSD3B1 Antibody (N-term)
Catalog Number: orb1935675
| Catalog Number | orb1935675 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB34568 |
| Tested applications | WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:1000 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 42252 Da |
| Target | This HSD3B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human HSD3B1. |
| UniProt ID | P14060 |
| NCBI | NP_000853.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-is Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
HSD3B1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35 ug/lane). This demonstrates the HSD3B1 antibody detected the HSD3B1 protein (arrow).
Western blot analysis of HSD3B1 (arrow) using rabbit polyclonal HSD3B1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HSD3B1 gene.
Anti-HSD3B1 Antibody (N-term) at 1:1000 dilution + human placenta lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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