HPV16 E6 + HPV18 E6 Rabbit Polyclonal Antibody

• Catalog Number: orb10840
• Category: Antibodies
• Description: HPV16 E6 + HPV18 E6 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Virus
• Reactivity: Human, Virus
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HPV16 E6+ HPV18 E6
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 11 kDa
• Target: E6
• RRID: AB_10750523
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: HPV16+HPV18 E6; HPV16/18 protein; Human Papilloma
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-HPV16 E6 + HPV18 E6 antibody (orb10840), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Recombinant HPV16 E6 protein, DsbC & His 0.1 ug, Lane 2: Recombinant HPV16 E6 protein, DsbC & His 0.05 ug, Primary: Anti-HPV16 E6 + HPV18 E6 (orb10840) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 11 kDa, Observed band size: 50 kDa.

Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-HPV16 E6+HPV18 E6 Polyclonal Antibody, Unconjugated (orb10840) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.