HPV-16 L1 Antibody

• Catalog Number: orb606483
• Category: Antibodies
• Description: Reacts with a protein of 57kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16). It is the major capsid protein of HPV-16. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies. The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33; very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: HPV16/2058R
• Tested applications: IHC-P
• Reactivity: Virus
• Isotype: Rabbit IgG, kappa
• Immunogen: Human papilloma virus type 16, major capsid protein L1, was used as the immunogen for the recombinant HPV16 L1 antibody.
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant HPV16 L1 antibody is available for research use only.
• UniProt ID: P03101
• Storage: Store the recombinant HPV16 L1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The stated application concentrations are suggested starting points. Titration of the recombinant HPV16 L1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human cervix with recombinant HPV16 L1 antibody (clone HPV16/2058R). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant HPV16 L1 antibody (clone HPV16/2058R) as confirmation of integrity and purity.