HPV-16 E6 Antibody

• Catalog Number: orb640113
• Category: Antibodies
• Description: Reacts with E6 protein of human papilloma virus type 16 (HPV-16). Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies. The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HPV16/1295
• Tested applications: IHC-P
• Reactivity: Virus
• Isotype: Mouse IgG1, kappa
• Immunogen: Amino acids MHQKRTAMFQDPPQERPRKLPQLC were used as the immunogen for this HPV-16 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This HPV-16 E6 antibody is available for research use only.
• Storage: Store the HPV-16 E6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human cervix with HPV-16 E6 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free HPV-16 E6 antibody (clone HPV16/1295) as confirmation of integrity and purity.