HP Antibody

• Catalog Number: orb1259375
• Category: Antibodies
• Description: HP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HP (NP_005134.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 45kDa
• Target: HP
• UniProt ID: P00738
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: HP, hp2-alpha, BP, HPA1S, HP2ALPHA2
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HP antibody (orb1259375) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat liver using HP antibody (orb1259375) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using HP antibody (orb1259375) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using HP antibody (orb1259375) at dilution of 1:100 (40x lens).