HOXB2 Antibody

• Catalog Number: orb2635059
• Category: Antibodies
• Description: Hox genes play a fundamental role in the development of the vertebrate central nervous system, heart, axial skeleton, limbs, gut, urogenital tract and external genitalia. The homeobox gene HoxB1 is critical to hindbrain development and has phenotypic features frequently observed in autism. Analysis of expression and targeted disruption of HoxB1 demonstrates that it is also essential for patterning progenitor cells along the entire DV axis of rhombomere 4 (r4). HoxB1 maintains this function by acting very early during hindbrain neurogenesis to specify effectors of the Sonic hedgehog and Mash1 signaling pathways. HoxB2 is a homeodomain protein important in neural development that is also expressed during erythropoiesis, hindbrain development and normal human adult lung development. HoxB2 may modulate the amount of gamma-globin mRNA expressed during development and differentiation. In addition, HoxB2 plays an important role in the patterning of hindbrain and pharyngeal arches in the zebrafish.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-HOXB2-1F2
• Tested applications: ELISA, FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: A portion of amino acids 114-220 was used as the immunogen for the HOXB2 antibody.
• Dilution range: ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This HOXB2 antibody is available for research use only.
• UniProt ID: P14652
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the HOXB2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human thyroid tissue with HOXB2 antibody (clone PCRP-HOXB2-1F2) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human breast carcinoma tissue with HOXB2 antibody (clone PCRP-HOXB2-1F2) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

FACS staining of PFA-fixed human HeLa cells with HOXB2 antibody (blue, clone PCRP-HOXB2-1F2) and isotype control (red).

Immunofluorescent staining of PFA-fixed human HeLa cells using HOXB2 antibody (green, clone PCRP-HOXB2-1F2) and phalloidin (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using HOXB2 antibody (clone PCRP-HOXB2-1F2). These results demonstrate the foremost specificity of the PCRP-HOXB2-1F2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.