HOXA10 Antibody

• Catalog Number: orb1248341
• Category: Antibodies
• Description: HOXA10 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Porcine
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: PQATSCSFAQNIKE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37-38kDa band observed in Human and Mouse Skeletal Muscle lysates (calculated MW of 42.4kDa according to Human NP_061824.3 and 43.2kDa according to Mouse NP_032289.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Testis shows nuclear staining preferentially in the spermatocytes and spermatids. Recommended concentration, 5-10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 38kDa
• Target: HOXA10
• UniProt ID: P31260
• NCBI: NP_061824.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: HOXA10, homeobox A10, HOX1, HOX1.8, HOX1H, PL, hom
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37-38kDa band observed in Human and Mouse Skeletal Muscle lysates (calculated MW of 42.4kDa according to Human NP_061824.3 and 43.2kDa according to Mouse NP_032289.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Testis shows nuclear staining preferentially in the spermatocytes and spermatids. Recommended concentration, 5-10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248341 (0.2 ug/ml) staining of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248341 (5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248341 (0.3 ug/ml) staining of Mouse Skeletal Muscle lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.