Horse IgG (H&L) antibody (Peroxidase)

• Catalog Number: orb347140
• Category: Antibodies
• Description: Horse IgG (H&L) antibody (Peroxidase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Equine
• Isotype: IgG
• Immunogen: Horse IgG whole molecule
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:60,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:20,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Horse IgG and Horse Serum.
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: goat Anti-Horse IgG peroxidase Conjugated Antibody
• Note: For research use only
• Application notes: Anti-Horse IgG Peroxidase Antibody has been tested by ELISA and is designed for western blotting and Immunohistochemistry.
• Expiration Date: 12 months from date of receipt.

ELISA Results of Goat Anti-Horse IgG Antibody Peroxidase Conjugated tested against purified Horse IgG. Each well was coated in duplicate with 1.0 µg of Horse IgG. The working dilution is 1:400000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Horse IgG HRP conjugated and TMB substrate (p/n orb348651).