Horse IgG F(c) fragment Antibody

• Catalog Number: orb346177
• Category: Proteins
• Description: Horse IgG F(c) fragment Antibody
• Tested applications: ELISA, IHC, SDS-PAGE, WB
• Isotype: IgG F(c)
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
• Form/Appearance: Liquid (sterile filtered)
• Purity: Horse IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Horse IgG F(c) fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse Serum, anti-Horse IgG and anti-Horse IgG F(c). No reaction was observed against anti-Horse IgG F(ab’)2 or anti-Papain.
• Conjugation: Unconjugated
• Source: Horse
• Biological Origin: Horse
• Storage: Store vial at 4° C prior to restoration. Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Horse IgG F(c) fragment is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: Horse immunoglobulin G F(c), IgG F(c), Horse Fc Fr
• Note: For research use only
• Application notes: Horse IgG F(c) Fragment has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
• Expiration Date: 12 months from date of receipt.

SDS-Page of Horse IgG F(c) Fragment. Lane 1: Horse IgG F(c) Fragment – Reduced. Lane 2: Horse IgG F(c) Fragment – Non-reduced. Load: 1.0 µg per lane. Predicted/Observed Size: Reduced- 25 kDa, Non-Reduced- 50 kDa.