Horse IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated

• Catalog Number: orb347166
• Category: Antibodies
• Description: Horse IgG F(ab')2 antibody (Alkaline Phosphatase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Equine
• Isotype: IgG
• Immunogen: Horse IgG F(ab')2 fragment
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:8,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Horse IgG, Horse IgG F(ab')2 and Horse Serum. No reaction was observed against Horse IgG F(c).
• Conjugation: AP
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Alternative names: Rabbit Anti-Horse IgG Fab2 Fragment alkaline phosp
• Note: For research use only
• Application notes: Anti-Horse IgG F(ab')2 Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

ELISA Results of Rabbit Anti-Horse IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated tested against purified Horse IgG F(ab')2 Alk Phos. Each well was coated in duplicate with 1.0 µg of Horse IgG F(ab')2 (p/n orb2652757). The working dilution is 14700. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Alkaline Phosphatase ELISA Substrate Buffer and NPP Working Buffer.