Horse IgG F(ab')2 antibody (Alkaline Phosphatase)

• Catalog Number: orb347145
• Category: Antibodies
• Description: Horse IgG F(ab')2 antibody (Alkaline Phosphatase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Equine
• Isotype: IgG
• Immunogen: Horse IgG F(ab')2 fragment
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Horse IgG, Horse IgG F(ab’)2 and Horse Serum. No reaction was observed against Horse IgG F(c).
• Conjugation: AP
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat Anti-Horse IgG Fab2 Fragment alkaline phospha
• Note: For research use only
• Application notes: Anti-Horse IgG F(ab')2 Alkaline Phosphatase Antibody has been tested by ELISA. This product has been assayed against 1.0 µg of Horse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.