Horse IgG Antibody

• Catalog Number: orb346176
• Category: Proteins
• Description: Horse IgG Antibody
• Tested applications: ELISA, IHC, SDS-PAGE, WB
• Isotype: IgG
• Concentration: 11.0 mg/mL
• Dilution range: ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
• Form/Appearance: Lyophilized
• Purity: Horse IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and extensive dialysis against the buffer stated above. Horse IgG whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse IgG, and anti-Horse Serum.
• Conjugation: Unconjugated
• Source: Horse
• Biological Origin: Horse
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Horse IgG whole molecule is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: Horse immunoglobulin G
• Note: For research use only
• Application notes: House IgG whole molecule has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
• Expiration Date: 12 months from date of receipt.

SDS PAGE of Horse IgG Whole Molecule. Lane 1: Non-Reduced Horse IgG Whole Molecule. Lane 2: 5 µL Opal Prestained Marker. Lane 3: Reduced Horse IgG Whole Molecule. Load: 1 µg per lane. Predicted/Observed size: Non-Reduced at 160 kDa, Observed at greater than 180; Reduced at 55, 25 kDa. Non-reduced sample migrates higher than predicted size.