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Catalog Number | orb546378 |
---|---|
Category | Antibodies |
Description | HOOK3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human HOOK3 recombinant protein (Position: E14-R198). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 83 kDa |
UniProt ID | Q86VS8 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Protein Hook homolog 3; h-hook3; Hhk3; HOOK3 Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U87 cells using anti-HOOK3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of HOOK3 using anti-HOOK3 antibody.Lane 1:human MOLT-4 cell;2:human RT4 cell;3:human SiHa cell;4:rat brain tissue;5:mouse brain tissue.
IF analysis of HOOK3 using anti-HOOK3 antibody. HOOK3 was detected in immunocytochemical section of A431 cells.
IHC analysis of HOOK3 using anti-HOOK3 antibody.HOOK3 was detected in paraffin-embedded section of human placenta tissue.
IHC analysis of HOOK3 using anti-HOOK3 antibody.HOOK3 was detected in paraffin-embedded section of mouse testis tissue.
IHC analysis of HOOK3 using anti-HOOK3 antibody.HOOK3 was detected in paraffin-embedded section of human glioma tissue.
IHC analysis of HOOK3 using anti-HOOK3 antibody.HOOK3 was detected in paraffin-embedded section of human oesophagus squama cancer tissue.
IHC analysis of HOOK3 using anti-HOOK3 antibody.HOOK3 was detected in paraffin-embedded section of human sarcoma tissue.
IHC analysis of HOOK3 using anti-HOOK3 antibody.HOOK3 was detected in paraffin-embedded section of rat testis tissue.
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Goat, Guinea pig, Mouse, Porcine, Rabbit, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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