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HO-1 (Rat) Antibody: HRP
Catalog Number: orb376896
| Catalog Number | orb376896 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against HO-1 conjugated to HRP. |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | 6B8-2F2 |
| Tested applications | ELISA, IHC, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG1 Kappa |
| Immunogen | His-tagged Rat HO-1 |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000), ICC/IF (1:100) |
| Conjugation | HRP |
| MW | 32kDa |
| Target | HO-1 |
| Entrez | 24451 |
| UniProt ID | P06762 |
| NCBI | NP_036712.1 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O |
| Alternative names | HSP32 antibody, HMOX1 antibody, Heme oxygenase 1 a Read more... |
| Note | For research use only |
| Application notes | 1 µg/ml of SMC-234 was sufficient for detection of HO-1 in 10 µg of rat kidney lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HO-1 (Rat) Monoclonal Antibody, Clone 6B8-2F2. Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HO-1 (Rat) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.
Western Blot analysis of Human, Mouse, Rat Rat Kidney Lysate showing detection of ~31 kDa HO-1 protein using Mouse Anti-HO-1 Monoclonal Antibody, Clone 6B8-2F2. Lane 1: MW Ladder. Lane 2: Rat Kidney Lysate. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~31 kDa.
