HNRNPA2B1 Antibody

• Catalog Number: orb1248243
• Category: Antibodies
• Description: HNRNPA2B1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-DTIEIITDRQS
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in lysates of cell line MCF7 and approx. 35kDa in lysates of cell line NIH3T3 and nuclear NIH3T3 (calculated MW of 36.0kDa according to NP_002128.1). An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.01-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Small Intestine shows nuclear staining. Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 36kDa
• Target: HNRNPA2B1
• UniProt ID: P22626
• NCBI: NP_112533.1, NP_002128.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: HNRNPA2B1, heterogeneous nuclear ribonucleoprotein
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in lysates of cell line MCF7 and approx. 35kDa in lysates of cell line NIH3T3 and nuclear NIH3T3 (calculated MW of 36.0kDa according to NP_002128.1). An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.01-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Small Intestine shows nuclear staining. Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
• Expiration Date: 12 months from date of receipt.

orb1248243 (0.3 ug/ml) staining of MCF7 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248243 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248243 (5 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248243 (0.1 ug/ml) staining of NIH3T3 (A), (0.01 ug/ml) nuclear NIH3T3 (B) lysate, + Peptide (C) (35 ug protein in RIPA buffer). Detected by chemiluminescence.