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Catalog Number | orb1520146 |
---|---|
Category | Antibodies |
Description | hnRNP M3/4 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | IP, WB |
Reactivity | Human, Mouse |
Immunogen | hnRNP M1-4 proteins |
Concentration | 40 µg/ml |
Dilution range | WB - 1:1000; IP - 20 µl/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | hnRNP M3/4 |
UniProt ID | P52272 |
NCBI | NP_005959.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | HNRPM; NAGR1; N-acetylglucosamine receptor 1; HTGR Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of mouse hnRNP M3/4 by western blot. Samples: Whole cell lysate (10 µg) from BW5147.3, CT26, CH27, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Mouse anti-hnRNP M3/4 monoclonal antibody [2A6-2H3] (orb1520146) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human hnRNP M3/4 by western blot. Samples: Whole cell lysate (5 µg) from HEK293T, LNCaP, A-549, MCF-7, and K-562 cells prepared using NETN lysis buffer. Antibody: Mouse anti-hnRNP M3/4 monoclonal antibody [2A6-2H3] (orb1520146) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human hnRNP M3/4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Mouse anti-hnRNP M3/4 monoclonal antibody [2A6-2H3] (orb1520146) used for IP at 20 µl/mg lysate.
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