hnRNP M3/4 Antibody

• Catalog Number: orb1520144
• Category: Antibodies
• Description: hnRNP M3/4 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: hnRNP M1-4 proteins
• Concentration: 40 µg/ml
• Dilution range: WB - 1:1000; IP - 20 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: hnRNP M3/4
• UniProt ID: P52272
• NCBI: NP_005959.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: HNRPM; NAGR1; N-acetylglucosamine receptor 1; HTGR
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human hnRNP M3/4 by western blot. Samples: Whole cell lysate (5 µg) from HEK293T, LNCaP, A-549, MCF-7, and K-562 cells prepared using NETN lysis buffer. Antibody: Mouse anti-hnRNP M3/4 monoclonal antibody [2A6-2H3] (orb1520144) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse hnRNP M3/4 by western blot. Samples: Whole cell lysate (10 µg) from BW5147.3, CT26, CH27, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Mouse anti-hnRNP M3/4 monoclonal antibody [2A6-2H3] (orb1520144) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human hnRNP M3/4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Mouse anti-hnRNP M3/4 monoclonal antibody [2A6-2H3] (orb1520144) used for IP at 20 µl/mg lysate.