hnRNP H1 antibody

• Catalog Number: orb666917
• Category: Antibodies
• Description: Rabbit polyclonal antibody to hnRNP H1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide of human hnRNP H1. The exact sequence is proprietary.
• Dilution range: WB: 1-500-2000, IF: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HNRNPH1
• Entrez: 3187, 140931, 59013
• UniProt ID: O35737, P31943, Q8VHV7
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-HNRPH antibody, anti-HNRPH1 antibody, anti-He
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of hnRNP H1 expression in HeLa (A), mouse brain (B) whole cell lysates. (Predicted band size: 49 kD; Observed band size: 55 kD)

Immunofluorescent analysis of hnRNP H1 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.