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Catalog Number | orb1258136 |
---|---|
Category | Antibodies |
Description | HNMT Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IP, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human HNMT (NP_008826.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IF: 1:50 - 1:100IP: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 33kDa |
Target | HNMT |
UniProt ID | P50135 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | Histamine N-methyltransferase, HMT, HNMT Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:100IP: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using HNMT antibody (orb1258136) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using HNMT antibody (orb1258136). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of HT-29 cells using 1 ug HNMT antibody (orb1258136). Western blot was performed from the immunoprecipitate using HNMT antibody (orb1258136) at a dilution of 1:1000. Detection: ECL Enhanced Kit. Exposure time: 10s.
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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