HMGB1 antibody

• Catalog Number: orb195321
• Category: Antibodies
• Description: HMGB1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HMGB1. Please contact us for the exact immunogen sequence. The peptide is available as orb374832.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: WB: 1:5000, IF/ICC: 1:1000, IHC-P: 1:1000
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 24 kDa
• Target: HMGB1
• Entrez: 3146
• UniProt ID: P63158, P09429, P63159
• NCBI: 002119, 11, 41, 002128
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti Amphoterin antibody, anti Chromosomal protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

F Li, N Qin, Y Yu, R Dong, X Li, S Gong, Z Zeng, L Huang, H Yang TREM-1 inhibition or ondansetron administration ameliorates NLRP3 inflammasome and pyroptosis in traumatic brain injury-induced acute lung injury Intensive Care Medicine, (2024)

PubMed:

Applications

WB

Reactivity

Rat

WB analysis of rat kidney (Lane 1), brain (Lane 2) using HMGB1 antibody (Dilution at 1:5000)

Immunofluorescence analysis of mouse sp20 mouse myeloma cells tissue using anti-HMGB1 (dilution of primary antibody - 1:300)

IHC-P staining of mouse skin tissue using HMGB1 antibody (dilution at 1:300)

Immunofluorescence image of mouse sp23 mouse myeloma cells tissue using anti-HMGB1 (dilution at 1:300)

Immunofluorescence image of mouse sp22 mouse myeloma cells tissue using HMGB1 antibody (dilution at 1:1000)

IF image of rat skin tissue using HMGB1 antibody (primary antibody at 1:1000)

Immunofluorescence image of rat skin tissue using anti-HMGB1 (dilution at 1:1000)

Immunofluorescence image of mouse sp22 mouse myeloma cells tissue using anti-HMGB1 (dilution at 1:300)

IHC-P staining of rat skin tissue using anti-HMGB1 (dilution at 1:1000)

IF analysis of mouse sp21 mouse myeloma cells tissue using HMGB1 antibody (dilution of primary antibody at 1:300)

Immunofluorescence analysis of rat skin tissue using HMGB1 antibody (dilution of primary antibody - 1:1000)

Immunohistochemical staining of paraffin embedded mouse brain tissue using HMGB1 antibody (primary antibody at 1:1000)

Immunofluorescence analysis of mouse sp21 mouse myeloma cells tissue using anti-HMGB1 (dilution of primary antibody - 1:1000)

IF image of rat skin tissue using anti-HMGB1 (primary antibody at 1:1000)

Immunohistochemical staining of rat skin tissue using HMGB1 antibody (dilution of primary antibody - 1:300)

Western blot analysis of rat kidney (Lane 1), rat brain (Lane 2) using HMGB1 antibody (dilution at 1:200)