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Catalog Number | orb43626 |
---|---|
Category | Antibodies |
Description | Mouse Monoclonal to HLA-G. |
Clonality | Monoclonal |
Clone Number | 87G |
Tested applications | FC |
Reactivity | Human |
Isotype | Mouse IgG2a |
Immunogen | HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin. |
Concentration | 1 mg/ml |
Dilution range | Flow cytometry: Extracellular and intracellular staining; recommended dilution: 2 μg/ml; positive control: JEG-3 human choriocarcinoma epithelial cell line. |
Purity | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | FITC |
Target | HLA-G |
Entrez | 3135 |
UniProt ID | P17693 |
RRID | AB_10993742 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-HLA-G antibody Read more... |
Note | For research use only |
Application notes | Flow cytometry: Extracellular and intracellular staining; recommended dilution: 2 μg/ml; positive control: JEG-3 human choriocarcinoma epithelial cell line. |
Expiration Date | 12 months from date of receipt. |
Separation of cells stained using anti-human HLA-G (87G) FITC antibody (concentration in sample 5 μg/ml, red-filled) from cells stained using mouse IgG2a isotype control (MOPC-173) FITC antibody (concentration in sample 5 μg/ml, same as HLA-G FITC concentration, black-dashed) in flow cytometry analysis (surface staining) of HLA-G transfected cells.
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