HLA-DRB1 Antibody (MHC II)

• Catalog Number: orb749570
• Category: Antibodies
• Description: This mAb reacts with the beta-chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Its epitope is different from that of mAb L243. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HLA-DRB/1067
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DRB1 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 2-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This HLA-DRB1 antibody is available for research use only.
• UniProt ID: P01911
• Storage: Store the HLA-DRB1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the HLA-DRB1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot Anaysis of human 1) Ramos and 2) spleen cell Lyste using HLA-DRB1 antibody (clone HLA-DRB/1067). Predicted molecular weight ~30 kDa.

Western blot Anaysis of Ramos cell Lyste using HLA-DRB1 antibody (HLA-DRB/1067). Predicted molecular weight ~30 kDa.

IHC: Formalin-fixed, paraffin-embedded human Histiocytoma stained with HLA-DRB antibody (clone HLA-DRB/1067).

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with HLA-DRB antibody (clone HLA-DRB/1067).

Immunofluorescent staining of Raji cells with HLA-DRB1 antibody (green, clone HLA-DRB/1067) and Reddot nuclear stain (red).

FACS staining of Raji cells with HLA-DRB1 antibody (clone HLA-DRB/1067); Red=isotype control, Blue=HLA-DRB1 antibody.