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Catalog Number | orb2276406 |
---|---|
Category | Antibodies |
Description | HLA-DRa antibody validated for immunohistochemistry on 76 different Normal Tissues |
Species/Host | Rabbit |
Clonality | Monoclonal |
Clone Number | Biorbyt-470R |
Tested applications | IHC |
Reactivity | Human |
Isotype | IgG |
Dilution range | 1:100-1:200 |
Conjugation | Unconjugated |
UniProt ID | P01903 |
Storage | Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required. |
Alternative names | DR alpha chain; DRB1; DRB4; HLA class II histocomp Read more... |
Note | For research use only |
Application notes | Positive Control: Liver: An at least moderate staining should be seen of sinusoids and Kupffer cells. Kidney: An at least moderate staining should be seen in small capillaries of glomeruli. Negative Control: Absence of HLA-DRa staining is expected in hepatocytes of a completely normal liver and in tubuli of completely normal kidney. Cellular Localization: Cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions. |
Expiration Date | 12 months from date of receipt. |
Colon HLA DR negative colorectal adenocarcinoma showing strong HLA DR positivity of capillaries and tumor associated inflammatory cells.
Liver: Sinusoids and Kupffer cells show strong HLA-DRa immunostaining in the liver.
Vulva Squamous cell carcinoma showing strong HLA DR immunostaining in tumor cells.
ELISA, FC, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
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