HLA-DRa antibody

• Catalog Number: orb2276405
• Category: Antibodies
• Description: HLA-DRa antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-470R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P01903
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: DR alpha chain; DRB1; DRB4; HLA class II histocomp
• Note: For research use only
• Application notes: Positive Control: Liver: An at least moderate staining should be seen of sinusoids and Kupffer cells. Kidney: An at least moderate staining should be seen in small capillaries of glomeruli. Negative Control: Absence of HLA-DRa staining is expected in hepatocytes of a completely normal liver and in tubuli of completely normal kidney. Cellular Localization: Cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Colon HLA DR negative colorectal adenocarcinoma showing strong HLA DR positivity of capillaries and tumor associated inflammatory cells.

Liver: Sinusoids and Kupffer cells show strong HLA-DRa immunostaining in the liver.

Vulva Squamous cell carcinoma showing strong HLA DR immunostaining in tumor cells.