HLA-DR Antibody (MHC II)

• Catalog Number: orb749565
• Category: Antibodies
• Description: mAb IPO-10 defines the antigen, which appears on B cell progenitors following HLA-DR and preceding CD10, CD19, CD22, CD37 and cym. It is expressed on resting B cells and than reappears and persists in cytoplasm and on cell surface until cytoplasmic Ig appears. It is a useful antibody for diagnostics of neoplasms of B cell origins. It reacts with human B cell lines Daudi, Raji, Namalva, EB-3, RPMI-8226 (50% of cells). The mAb does not label T cell lines, blood granulocytes, thymocytes or bone marrow stromal fibroblasts. No significant changes are detected after PHA or ConA stimulation while LPS and PWM stimulated cultures after 18-48h show decreased number of antigen-positive cells but in final terms of cultivation antigen is expressed again. This mAb labels B cell leukemias and some lymphomas. Hairy cell leukemia strongly reacts and 70% of B cell CLL and some B-NHL were also positive. IPO-10 reacts with AMML cells and in a majority of Hodgkin's disease cases a significant percentage of affected lymph node cells were detected.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: IPO-10
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG3, kappa
• Immunogen: Spleen cells from a patient with hairy cell leukemia were used as the immunogen for the HLA-DR antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This HLA-DR antibody is available for research use only.
• UniProt ID: P01903
• Storage: Store the HLA-DR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the HLA-DR antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE Analysis of Purified, BSA-Free HLA-DR Antibody (clone IPO-10). Confirmation of Integrity and Purity of the Antibody.