HLA-DQ Antibody (MHC II)

• Catalog Number: orb606900
• Category: Antibodies
• Description: Recognizes a DQ antigen, which is a dimer of 60kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: HLA-DQA1/2866R
• Tested applications: FACS, IF, IHC-Fr
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Full length recombinant human protein was used as the immunogen for the recombinant HLA-DQ antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant HLA-DQ antibody is available for research use only.
• UniProt ID: P01908, P01920, P01906
• Storage: Store the recombinant HLA-DQ antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant HLA-DQ antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of human Raji cells with recombinant HLA-DQ antibody (green, clone HLA-DQA1/2866R) and Reddot nuclear stain (red).

FACS testing of human Raji cells with recombinant HLA-DQ antibody (blue, clone HLA-DQA1/2866R) and isotype control (red).

SDS-PAGE analysis of purified, BSA-free recombinant HLA-DQ antibody (clone HLA-DQA1/2866R) as confirmation of integrity and purity.