HLA-DPB1 Antibody (MHC II)

• Catalog Number: orb606899
• Category: Antibodies
• Description: Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linked alpha (33-35kDa) and beta (27-30kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: HLA-DPB1/2862R
• Tested applications: ELISA, FACS, IF
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Non-T, non-B human acute lymphoblastic leukemia REH6 cell line was used as the immunogen for the recombinant HLA-DPB1 antibody.
• Dilution range: ELISA: 2-4ug/ml for coating (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant HLA-DPB1 antibody is available for research use only.
• UniProt ID: P04440
• Storage: Store the recombinant HLA-DPB1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant HLA-DPB1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of human Raji cells with recombinant HLA-DPB1 antibody (clone HLA-DPB1/2862R, green) and Reddot nuclear stain (red).

Flow cytometry testing of human Raji cells with recombinant HLA-DPB1 antibody; Red=isotype control, Blue= recombinant HLA-DPB1 antibody.

SDS-PAGE analysis of purified, BSA-free recombinant HLA-DPB1 antibody (clone HLA-DPB1/2862R) as confirmation of integrity and purity.