HLA-DP + HLA-DQ + HLA-DR Antibody (MHC II)

• Catalog Number: orb386111
• Category: Antibodies
• Description: This mAb reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some auto-immune diseases are associated with certain class II alleles.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CR3/43
• Tested applications: ELISA, FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Human tonsil cells were used as the immunogen for the HLA-DP/DQ/DR antibody.
• Dilution range: ELISA: 2-4ug/ml for coating (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/ million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This HLA-DP/DQ/DR antibody is available for research use only.
• UniProt ID: P01909, P01920, P01903, P04440, P01908
• Storage: Store the HLA-DP/DQ/DR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the HLA-DP/DQ/DR antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Western blot testing of human Ramos cell lysate with HLA-DP/DQ/DR antibody (clone CR3/43). Expected molecular weight ~36 kDa (alpha chain) and ~27 kDa (beta chain).

Western blot testing of human spleen cell lysate with HLA-DP/DQ/DR antibody (clone CR3/43). Expected molecular weight ~36 kDa (alpha chain) and ~27 kDa (beta chain).

IF/ICC staining of human Raji cells with HLA-DP/DQ/DR antibody (green) and Reddot nuclear stain (red).

Flow cytometry testing of human Raji cells with HLA-DP/DQ/DR antibody at 1ug/10^6 cells (blocked with goat sera); Red=isotype control, Blue=HLA-DP/DQ/DR antibody.

SDS-PAGE analysis of purified, BSA-free HLA-DP/DQ/DR antibody (clone CR3/43) as confirmation of integrity and purity.