HLA class II Antibody [F3.3], Rabbit IgG

• Catalog Number: orb1672144
• Category: Antibodies
• Description: HLA class II Antibody [F3.3], Rabbit IgG
• Clonality: Recombinant
• Clone Number: F3.3
• Tested applications: FC, WB
• Reactivity: Human
• Isotype: IgG kappa
• Concentration: batch dependent
• Conjugation: Unconjugated
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: HLA-DR, HLA class II related epitope
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot using anti-HLA class II histocompatibility antigen DR antibody F3.3. Human spleen (A)- human tonsil (B) and Daudi cell lysate (C) samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of F3.3 (orb1672144) at 0.01 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for HLA II DR is ~30 kDa- depending upon the exact DR isoform expressed (c.f. Klitz et al- PMID: 12974796). orb1672144 successfully detected human HLA class II histocompatibility antigen DR in human spleen and tonsil lysate as well as Daudi cell lysate.

Flow-cytometry using the anti-HLA II DR antibody F3.3. Daudi cells were stained with unimmunized r antibody (black line) or the rmeric version of F3.3 (orb1672144 - blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing- bound antibody was detected using anti-r JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Flow-cytometry using anti-HLA class II antibody F3.3. Human leukocytes were stained with an isotype control (3.0 - panel A) or the rmeric version of F3.3 (orb1672144 - panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody and cells analysed on a FACSCanto flow-cytometer.