Histone H4 (phospho-Ser47) antibody

• Catalog Number: orb767568
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H4 (phospho-Ser47)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H4 around the phosphorylation site of Ser47. AA range:13-62
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 13
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-HIST1H4A antibody, anti-H4/A antibody, anti-H
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HUVEC cells, using Histone H4 (Phospho-Ser47) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).