Histone H4 K12-Ac antibody

• Catalog Number: orb420436
• Category: Antibodies
• Description: Histone H4 K12-Ac antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, IF, IHC, Multiplex Assay, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Histone H4 [ac Lys12] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 12 of human Histone H4.
• Concentration: 0.69 mg/mL
• Dilution range: IHC: 1:100, IF: 1:10, WB: 1.0 ug/mL
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Histone H4 [ac Lys12] was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Alfalfa based on 100% sequence homology. Cross-reactivity with Histone H4 [ac Lys12] from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P62805
• NCBI: NP_001029249
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Histone H4 Ac Lys12 antibody, H4K12ac,
• Note: For research use only
• Application notes: Anti-Histone H4 K12 ac antibody is tested for Western Blot, Dot Blot, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~11 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus antibody production in collaboration with Novus Biologicals.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Rabbit Histone H4 K12-Ac (RABBIT) Antibody. Lane 1: K12 unmodified. Lane 2: K12-Acetylated. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H4 K12-Ac antibody at 1:1000 for 45 min at 4°C. Secondary antibody: RABBIT IgG (H&L) Secondary Antibody HRP Conjugated at 1:40000 for 30 min at RT. Block: 5% BLOTTO 30 minutes at RT.

Immunofluorescence of Histone H4 K12-Ac (RABBIT) Antibody: Histone H4 K12-Ac antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Western Blot of Histone H4 K12-Ac (RABBIT) Antibody. Western Blot analysis against untreated cell extracts. Lane 1: HeLa cell lysates. Load: 35 µg per lane. Primary antibody: Histone H4 K12-Ac antibody at 1.0 µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 11 kDa for Histone H4 K12-Ac (RABBIT) Antibody. Other band(s): none.