Histone H3(di Methyl K27) antibody

• Catalog Number: orb499667
• Category: Antibodies
• Description: Histone H3(di Methyl K27) antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5C11
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse, Rat
• CAS Number: Histone H3.1
• Isotype: IgG1
• Immunogen: KLH conjugated synthesised methylpeptide derived from human Histone H3 around the methylation site of di methyl K27 (AR(di Methyl-K)SA)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 15 kDa
• Target: Histone H3(di methyl K27)
• UniProt ID: P68431
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Histone H3 (di methyl Lys27); H3K27me2; H3 histone
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC-P analysis of Rat liver using Histone H3di methyl K27 Monoclonal Antibody at 1:400 dilution.

IHC-P analysis of Mouse colon using Histone H3di methyl K27 Monoclonal Antibody at 1:400 dilution.

IHC-P analysis of Human colon carcinoma using Histone H3di methyl K27 Monoclonal Antibody at 1:2000 dilution.

Western blot:Testis (Mouse);U2os(Mouse) Cell;Primary:Anti-Histone H3(di methyl K27) at 1:1000 dilution.

Western blot:MG63(Human) Cell;Primary:Anti-Histone H3(di methyl K27) at 1:1000 dilution.