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Catalog Number | orb1527686 |
---|---|
Category | Antibodies |
Description | Histone H3, Phospho (S10) Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IHC, WB |
Reactivity | Human, Mouse |
Immunogen | Surrounding serine 10 |
Concentration | 200 µg/ml |
Dilution range | WB - 1:5,000 - 1:10,000; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:2,000 - 1:10,000. Formaldehyde fixation and permeabilization with Triton-X 100 is recommended. Acetone fixation may adversely effect staining. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | Histone H3, Phospho (S10) |
UniProt ID | P68431 |
NCBI | NP_003520.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | Histone H3/c; Histone H3/k; Histone H3/l; Histone Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human Phospho Histone H3 (pS10) by western blot. A-549, HeLa, K-562, and Jurkat cell lines were stimulated with 0.5 µg/ml of Calyculin A at 37°C, 5% CO₂ for 15 minutes. Cells were subsequently lysed and 1 µg of appropriate cell lysates were loaded into respective wells. Western blot was performed using affinity purified rabbit anti-Histone H3 (pS10) antibody orb1527686 (20 ng/ml) and affinity purified rabbit anti-Histone H3 antibody (1 µg/ml). Western blots were developed utilizing chemiluminescence with an exposure time of 30 seconds.
Detection of human and mouse Histone H3 (S10) by immunohistochemistry.Sample: FFPE section of human colon carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) (Cat. No. orb1527686) used at a dilution of 1: 2, 500 (0.08 µg/ml). Detection: DAB
Detection of human Phospho Histone H3 (S10) by immunocytochemistry. Samples: NBF-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-Phospho Histone H3 (S10) (Cat. No. orb1527686) used at a dilution of 1: 2, 500 (0.08µg/ml). Detection: Green fluorescent goat antirabbit IgG-FITC conjugated used at a dilution of 1: 100.
IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Porcine, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IP, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
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